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C2724
Sigma
Anti-Calbindin-D-28K (EG-20) antibody produced in rabbit
affinity isolated antibody, buffered aqueous solution
| MDL number: | MFCD00164553 |
Description
| Analysis Note | Enzymatic predigestion of formalin-fixed, paraffin-embedded sections by proteolytic enzymes (e.g., 0.1% trypsin or protease, 10 min at RT or 37 °C) improves immunohistochemical staining. |
| Immunogen | synthetic peptide corresponding to the C-terminal region of rat calbindin-D-28K (amino acids 225-244 with N-terminally added Lys-Gly) conjugated to KLH. |
| Specificity | The immunogen sequence is specific for calbindin-D-28K and is not found in other members of the EF-hand family such as calbindin-D-9K, calretinin, myosin light chain, parvalbumin, S-100a, S-100b, S100A2 (S100L), and S100A6 (calcyclin). This sequence is highly conserved across species, e.g., in the corresponding human, mouse, bovine, chicken, porcine, and frog calbindin-D-28K sequences. |
| Physical form | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide. |
Properties
| antibody form | affinity isolated antibody |
| form | buffered aqueous solution |
| mol wt | antigen mol wt 28 kDa |
| species reactivity | rat |
| application(s) | immunoblotting: 1:2,000 using a whole extract of rat brain |
| immunohistochemistry: 1:2,000 using sections of rat cerebellum | |
| Gene Information | rat ... Calb1(83839) |
| shipped in | dry ice |
| storage temp. | −20°C |
Safety
| WGK Germany | NWG |
References
| Reference | Johnson, K., et al., Postnatal phencyclidine administration selectively reduces adult cortical parvalbumin-containing interneurons. Neuropsychopharmacology 33, 2442-2455, (2008) Abstract |
| Meyer, E.A., et al., Differences in Chemo- and Cytoarchitectural Features within Pars Principalis of the Rat Anterior Olfactory Nucleus Suggest Functional Specialization J. Comp. Neurol. 498, 786-795, (2006) Abstract | |
| Gargini, C., et al., Retinal organization in the retinal degeneration 10 (rd10) mutant mouse: a morphological and ERG study. J. Comp. Neurol. 500, 222-238, (2007) Abstract |
